I agree with Biffo that you'd be not likely to confuse "No." While using the word "No" at the time you consider it. But I do sometimes encounter a break up-second hesitation when reading through a sentence with "No.", particularly if there's a Room among it and the subsequent variety: https://manueletgsx.myparisblog.com/37101621/the-definitive-guide-to-no-scrub-cover