As Vp was set and E0 didn't alter, channel conductance will need to have reduced. Moreover, in the skin-out patches, the same gradual reduction in conductance was recorded. It's not at all inconceivable that bicuculline, when it binds towards the GABA-binding web-site, could alter the conformation on the channel. A https://archerthuoa.uzblog.net/tcep-hydrochloride-for-dummies-43063646